Spontaneous induction of cryptic prophages in populations of the model species Corynebacterium glutamicum and Escherichia coli

Spontaneous prophage induction in a small subpopulation of cells which takes place in the absence of a known stimulus is an often observed, but poorly understood phenomenon. With the proposed project we aim to investigate the impact of stress responses and stochasticity fluctuations of key regulatory proteins on the spontaneous induction of the Corynebacterium glutamicum prophage CGP3 at the single cell level. We aim to combine classical microbiological approaches with stochastic modelling and the design of novel microfluidic devices for single cell studies to contribute to a better understanding of spontaneous prophage induction as a general phenomenon in bacterial populations.

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Contact details:
PI Microbiology
Prof. Dr. Julia Frunzke
Forschungszentrum Jülich
Institut für Bio- und Geowissenschaften
IBG-1: Biotechnologie
D-52425 Jülich
Tel.: +49-(0)2461-61-5430
Fax: +49-(0)2461-61-3953
 j.frunzke@fz-juelich.de
Homepage Link
PIs Modelling
Prof. Dr. Wolfgang Wiechert
Forschungszentrum Jülich
Institut für Bio- und Geowissenschaften
IBG-1: Biotechnologie
D-52425 Jülich
Tel.: +49-(0)2461-61-5557
Fax: +49-(0)2461-61-3870
 w.wiechert@fz-juelich.de
Homepage Link
Dr. Katharina Nöh
Forschungszentrum Jülich
Institut für Bio- und Geowissenschaften
IBG-1: Biotechnologie
D-52425 Jülich
Tel.: +49-(0)2461-61-9294
Fax: +49-(0)2461-61-3870
 k.noeh@fz-juelich.de
Homepage Link
PI Microfluidics
Junior Prof. Dr. Dietrich Kohlheyer
Forschungszentrum Jülich
Institut für Bio- und Geowissenschaften
IBG-1: Biotechnologie
D-52425 Jülich
Tel.: +49-(0)2461-61-2875
Fax: +49-(0)2461-61-3870
 d.kohlheyer@fz-juelich.de
Homepage Link
Co-workers: PhD Students: Arun Nanda, Stefan Helfrich